Jonathan M. Green, MD

Assistant Professor of Medicine & Pathology

CONTACT INFORMATION

Washington University School of Medicine
660 S. Euclid Ave., Campus Box 8052
St. Louis, MO 63110
(314) 747-3591 (phone)
jgreen@im.wustl.edu

EDUCATION/TRAINING

University of Michigan, Ann Arbor, MI B.S. 1982 Microbiology
Wayne State University, Detroit, MI M.D. 1986 Medicine
Boston City Hospital, Boston, MA Internship 1987 Internal Medicine
Boston City Hospital, Boston, MA Residency 1989 Internal Medicine

POSITIONS

1989 - 1990 Research Fellow, Pulmonary Center, Boston University School of Medicine, Boston, MA. Mentor: J. Berman M.D.
1990 - 1993 Postdoctoral Fellow, Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI. Chief Galen Toews, M.D.
1991 - 1996 Postdoctoral Research Fellow, University of Michigan/University of Chicago, Chicago, IL. Mentor: Craig B. Thompson, M.D.
1995 - 1996 Research Associate (Instructor), Department of Medicine, Univ. of Chicago, Chicago, IL.
1996 - Assistant Professor of Medicine and Pathology and Immunology, Washington University, St. Louis, MO

HONORS

1984 First Place in Annual Medical Student Research Award, Wayne State University
1986 Distinction in Biomedical Research Award, Wayne State University
1991 - 1993 American Lung Association Research Training Fellowship
1993 - 1995 Individual National Research Service Award (F32-AI09042)
1995 - 1996 Gaylord Donnelly Award, American Lung Association of Metropolitan Chicago
1998 - 1999 HHMI/Washington University Pilot project award

PUBLICATIONS

Green, J., and Maisel, H. Lens fodrin binds actin and calmodulin. 1984. Curr. Eye Res. 12:1433-1440.

Green, J.M., Turka, L.A., June, C.H., and Thompson, C.B. CD28 and staphylococcal enterotoxin synergize to induce MHC independent T-cell proliferation. 1992. Cell. Immunol. 145:11-20

Mao, X., Green, J.M., Safer, B., Lindsten, T., Frederickson, R.M., Miyamoto, S., Sonenburg, N., and Thompson, C.B. Regulation of translation initiation factor gene expression during human T cell activation. 1992. J. Biol. Chem. 267(28):20444-20450

Nickoloff, B.J., Mitra, R.S., Lee, K., Turka, L.A., Green, J., Thompson, C., and Shimizu, Y. Discordant expression of CD28 ligands, BB-1 and B7 on keratinocytes in-vitro and psoriatic cells in-vivo. 1993. Am. J. Path. 142(4):1029-40

Nickoloff, B.J., Mitra, R.S., Green, J.M., Zheng, X-G, Shimizu, Y., Thompson, C.B., and Turka, L.A. Accessory cell function of keratinocytes for superantigens: dependence on LFA-1/ICAM-1 interactions. 1993. J. Immunol. 150(6):2148-59

Turka, L.A, Gratiot-Deans, J., Kiem, D., Bandukwala, R., Green, J.M., Strahler, J. and Hanash, S.M. Elevated PCNA levels in immature thymocytes: dissociation from cell cycle progression. 1993 J. Immunol. 150(7):2746-52

Samet, J.H., Burstin, H.R., Green, J.M., and Singer, D.E. Sociodemographic determinants in the hospitalization decision: evaluation of an emergency department interhospital transfer policy. 1993. Ann. Emerg. Med. 22(5):813-8

Nestle, F.O., Mitra, R.S., Green, J., Shimizu, Y., Thompson, C., Turka, L.A., Activated keratinocytes present bacterial-derived superantigens to T lymphocytes: relevance to psoriasis. 1994. J. Derm Science. 6:127-133.

Green, J.M., Zheng, X., Shimizu, Y., Thompson, C.B. and Turka, L.A. T cell receptor stimulation, but not CD28 costimulation, is dependent on LFA-1 mediated events. 1994. Eur. J. Immunol. 24:265-272

Goodman, R.E., Nestle, F., Naidu, Y.M., Green, J.M., Thompson, C.B., Nickoloff, B.J. and Turka, L.A. Keratinocyte-derived T cell costimulation induces preferential production of IL-2 and IL-4 but not interferon-g. 1994. J. Immunol 152:5189-5198

Walunas, T.L., Lenschow, D.J., Bakker, C.Y., Linsley, P.S., Freeman, G.J., Green, J.M., Thompson, C.B. and Bluestone, J.A. CTLA-4 can function as a negative regulator of T cell activation. 1994. Immunity. 1:405-413

*Green, J.M., *Noel, P.J., Sperling, A.I., Walunas, T.L., Gray, G.S., Bluestone, J.A. and Thompson, C.B., Absence of B7-dependent costimulation in CD28-deficient mice. 1994. Immunity 1:508-513 (*co-first author)

*Green, J.M., *Sperling, A.I., Mosley, R.L., Smith. P.L., DiPaolo, R.J., Klein, J.R., Bluestone, J.A. and Thompson, C.B., CD43 is a murine costimulatory factor that functions independently of CD28. 1995. J. Exp. Med., 182:139-146. (*co-first author)

Kelleher, M.D., Abe, M.K., Chao, T., Jain, M., Green, J.M., Solway, J., Rosner, M.R. and Hershenson, M.B., Role of map kinase activation in bovine tracheal smooth muscle mitogenesis. 1995. Amer. J. Physiol. 268:L894-901.

Kim J.S., Rabe, K.F., Magnussen, H., Green, J.M., White, S.R., Migration and proliferation of guinea pig and human airway epithelial cells in response to tachykinins. 1995. Amer. J. Physiol., 269:L119-126

Ding, L., Green, J.M., Thompson, C.B. and Shevach, E.M. B7/CD28 dependent- and independent- pathways for induction of CD40 ligand (CD40L) expression. 1995. J. Immunol. 155:5124-5132

Noel, P.J., Boise, L.H., Green, J.M. and Thompson, C.B., CD28 costimulation is required to prevent cell death during the primary activation of T cells. 1996. J. Immunol. 157:636-642.

Green, J.M. and Thompson, C.B., Homotypic interactions mediated through LFA-1/ICAM-3 decrease the proliferative response of activated T cells. 1996. Cell. Immunol. 171:126-131

*Green, J.M., *Brown, D.R., Moskowitz, N.H., Davis, M., Thompson, C.B. and Reiner, S.L., Limited role for CD28-mediated signals in T helper subset differentiation. 1996. J. Exp. Med. 184:803-810 (*co-first author)

Vella, A.T., Groth, B., Linsley, P.S., Green, J.M., Thompson, C.B., Kappler, J.W. and Marrack, P. CD28 engagement and proinflammatory cytokines contribute to T cell proliferation and survival in vivo. 1996. J. Immunol. 158:4714-4720

Thurman, E.C., Walker, J., Jayaraman, S., Manjunath, N., Ardman, B. and Green, J.M. Regulation of in vitro and in vivo T cell activation by CD43. 1998. Int. Immunol. 10:691-701

Walker, J and Green, J.M. Structural requirements for CD43 function. 1999. J. Immunol. 162:4109-4114

Holdorf, A.D., Green, J.M., Levine, S.D., Straus, D.B., Link, V., Changelian, P.S., Allen, P.A. and Shaw, A.S. 1. Proline residues in CD28 and the SH3 domain of Lck are required for T cell costimulation. 1999. J. Exp. Med. 190:375-384

Green, J.M., Karpitskiy, V.S., Kimzey, S.L. and Shaw, A.S. 2000. Coordinate regulation of T cell activation by CD2 and CD28. J. Immunol. 164:3591-3595

Wang, W., Link, V.L. and Green, J.M., 2000. Identification and cloning of a CD43 associated serine/threonine kinase. 2000 Cell. Immunol. 205:34-39

Burr, J.S., Kimzey, S.K., Randolph, D.R. and Green, J.M. 2001. CD28 and CTLA4 coordinately regulate airway inflammatory cell recruitment and T helper cell differentiation in response to inhaled allergen. Amer. J. Resp. Cell. Mol. Biol. 24:563-568.

Burr, J.S., Savage, N.D.L., Kimsey, S.L. Messah, G.E., Shaw, A.S., Arch, R.H. and Green, J.M., 2001. Cutting Edge: Distinct motifs within CD28 regulate T cell proliferation and induction of Bcl-XL. J. Immunol. 166:5331-5335.

Bromley, S.K., Iaboni, A., Davis, S.J., Whitty, A., Green, J.M., Shaw, A.S., Weiss, A. and Dustin, M.L., 2001. The immunological synapse and CD28-CD80 interactions. Nature Immunology. 2:1159-1166

Savage, N.D.L., Kimzey, S.L., Bromley, S.K., Johnson, K.G., Dustin, M.L. and Green, J.M., 2001. Polar redistribution of the sialoglycoprotein CD43, implications for T cell function. J. Immunol. In Press

RESEARCH SUPPORT

ONGOING
RO1 HL58444 (Green) 09/01/97-07/31/02 NIH/NHLBI
Mechanism of CD43 Regulation of Pulmonary Inflammation
The goal of this project is to examine the mechanism by which CD43 regulates inflammatory processes in the lung.
Overlap: There is no scientific or budgetary overlap. This award was initially an R29, but has been converted to an RO1 to allow for a decrease in the percent of effort.
Role: Principal Investigator

RO1 HL62683 (Green) 02/15/00 Ü 01/31/04
NIH/NHLBI
Structural Basis of CD28 Regulation of Lung Inflammation
The goal of this project is to identify the structural elements of CD28 that regulate its function in vitro. Overlap: There is no scientific or budgetary overlap.
Role: Principal Investigator

Siteman Cancer Center (Green) 06/01/01 Ü 05/31/02
Development of lymphoma in CD2/CD28 double deficient mice
The goal of this project is to determine the incidence and kinetics of spontaneous lymphoma in CD2/CD28-double deficient mice, characterize the susceptibility of immunocompetent and immunodeficient hosts to transfer of CD2/CD28-deficient lymphoma cells, and to perform a preliminary biochemical analysis of spontaneously arising tumors in CD2/CD28-double deficient mice.
Overlap: There is no scientific or budgetary overlap.
Role: Principal Investigator

American Lung Association (Green) 07/01/01 Ü 06/30/02
T Cell Survival and Differentiation in response to Allergen
The goals of this project are to determine the relationship between proline mediated activation of Lck by CD28 and the recruitment and activation of Itk and PI-3 kinase, and to examine CD28 and CTLA4 mediated regulation of T cell function in allergen induced airway inflammation in vivo.
Overlap: There is no scientific or budgetary overlap. This grant will end prior to the startup of this application.
Role: Principal Investigator

COMPLETED
KO8 HL03408 (Green) 05/01/96 Ü 04/30/01
NIH
T Cell Costimulation in Inflammatory Lung Diseases
The goals of this project were to define the role of CD28 in airway inflammation using models of inflammatory lung disease and the CD28-deficient mouse.
Role: Principal Investigator

Division of Pulmonary and Critical Care Medicine
Department of Medicine
Washington University School of Medicine